endothelial cell adhesion molecule 1  (R&D Systems)

Journal: Materials Today Bio

Article Title: Delivered baicalein immunomodulatory hydrogel with dual properties of pH-responsive and anti-infection orchestrates pro-regenerative response of macrophages for enhanced hypertrophic scars therapy

doi: 10.1016/j.mtbio.2025.102160

Figure Lengend Snippet: Functional performance and biocompatibility evaluation of hydrogels. (A) Photographs of MRSA and E.coil colonies, and (B, C) corresponding quantitative calculation of antibacterial rates after treatments of different hydrogels. (D) Representative scratch assay images of L929 cells treated with different hydrogels and (E) corresponding quantitative analysis of cell migration rates based on scratch area. ELISA was performed to assess the expression of CD31 (F) and VEGF (G) in HUVEC cells following treatment with various hydrogels. (H) Photographs of hemolysis assays and (I) statistically analyzed hemolysis rates. (J) Cytocompatibility of hydrogels and in vivo hematological parameters, including (K)white blood cell (WBC) count -, (L)red blood cell (RBC) count , and (M)platelet count. n = 3, ∗P < 0.05, ∗∗P < 0.01 and ∗∗∗P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Following seeding in 12-well culture plates, HUVECs were maintained for 72 h in experimental media supplemented with different test materials, after which the supernatants were collected, centrifuged, and analyzed for CD31 (E-EL-H6227, Elabscience) and VEGF (E-EL-H0111, Elabscience) concentrations.

Techniques: Functional Assay, Wound Healing Assay, Migration, Enzyme-linked Immunosorbent Assay, Expressing, In Vivo